End-point Chromogenic Method for Bacterial Endotoxin Testing: Reliable Solutions from China Suppliers and Factory

End-point Chromogenic Method for Bacterial Endotoxin Testing (BET)
The End-point Chromogenic Method is a precise quantitative assay utilizing Limulus Amebocyte Lysate (LAL). This advanced technique allows for the detection of endotoxins by activating Factor C, which triggers an enzymatic cascade that cleaves a chromogenic substrate, leading to the release of a measurable chromophore. This product is not registered with the U.S. FDA and is intended for research use only within the U.S.
Procedure:

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1. Combine the test sample with LAL reagent and chromogenic substrate.
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2. Incubate the mixture at 37 ± 1 °C for a designated period, such as 60 minutes.
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3. Terminate the reaction with acid and measure the absorbance at 405 nm. Endotoxin levels are determined by comparing the absorbance of the sample against a standard curve established from known endotoxin concentrations. This method ensures high specificity and quantitative accuracy, making it a preferred choice for pharmaceutical and medical device testing. With a focus on providing high-quality testing solutions, our China suppliers and factory are committed to delivering reliable products for endotoxin testing.
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