End-point Chromogenic Method for Bacterial Endotoxin Testing: Reliable China Suppliers and Factory Solutions

End-point Chromogenic Method for Bacterial Endotoxin Testing (BET)
The End-point Chromogenic Method is a precise quantitative assay utilizing Limulus Amebocyte Lysate (LAL). This method involves endotoxin activating Factor C, which initiates an enzymatic cascade that cleaves a chromogenic substrate, resulting in the release of a measurable chromophore. Please note that this product is not registered with the U.S. FDA and is intended for research use only in the U.S.
Procedure:

1. Combine the test sample with LAL reagent and chromogenic substrate.
2. Incubate at 37 ± 1 °C for a specified duration (e.g., 60 min).
3. Stop the reaction using acid and measure the absorbance at 405 nm. Endotoxin levels are ascertained by comparing the sample’s absorbance to a standard curve formulated from known endotoxin concentrations. This method offers high specificity and quantitative accuracy, making it a preferred choice in pharmaceutical and medical device testing. As a leading supplier in China, our factory is dedicated to providing high-quality testing solutions tailored for your needs.