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KCA Endotoxin Assay Kit (Kinetic Chromogenic Method)

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KTA Endotoxin Assay Kit (Kinetic Turbidimetric Method)

KTA Endotoxin Assay Kit (Kinetic Turbidimetric Method)
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End-point Chromogenic Method for Bacterial Endotoxin Testing: China Suppliers & Factory Solutions

End-point Chromogenic Method for Bacterial Endotoxin Testing (BET)
The End-point Chromogenic Method is a precise quantitative assay that utilizes Limulus Amebocyte Lysate (LAL) to detect bacterial endotoxins. This innovative method works by having endotoxin activate Factor C, which initiates an enzymatic cascade that cleaves a chromogenic substrate, resulting in a measurable chromophore. Please note, this product is not registered with the U.S. FDA and is intended for research use only in the U.S.
Procedure:,
    ,
  1. Combine the test sample with LAL reagent and chromogenic substrate.
    2. ,
  2. Incubate the mixture at 37 ± 1 °C for a predetermined period (e.g., 60 minutes).
    3. ,
  3. Terminate the reaction with acid, then measure the absorbance at 405 nm. The endotoxin levels are calculated by comparing the sample's absorbance against a standard curve created from known endotoxin concentrations. This method is renowned for providing high specificity and quantitative accuracy, making it a preferred choice in pharmaceutical and medical device testing.
    As a leading manufacturer in China, we work with various suppliers to ensure quality and reliability in our products.
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    Product Details

    Catalog Number

    Catalog No. Description The kit Specification and Configuration
    ECA40TA BETMAT™ EC Endotoxin Assay Kit
    (End-point Chromogenic Method),
    40 Tests/Kit
    • 2 vials of LAL Reagent, 1.1ml/vial
    • 2 vials of LAL Reagent Water, 20ml/vial
    • 2 vials of Control Standard Endotoxin
    • 2 vials of Chromogenic Substrate, 2.2 ml/vial
    ECA40TB
    Frequently Asked Questions
    1. What is the detection method of the BETMAT™ EC Endotoxin Assay Kit?

    The kit utilizes the End-point Chromogenic Method for the quantitative detection of endotoxins.

    2. What catalog numbers are available for this product?

    The catalog numbers for this 40 Tests/Kit configuration are ECA40TA and ECA40TB.

    3. How many tests can be performed with one kit?

    Each BETMAT™ EC Endotoxin Assay Kit is configured to perform up to 40 tests.

    4. What is included in the kit configuration?

    The kit contains 2 vials of LAL Reagent (1.1ml/vial), 2 vials of LAL Reagent Water (20ml/vial), 2 vials of Control Standard Endotoxin, and 2 vials of Chromogenic Substrate (2.2 ml/vial).

    5. What are the volumes of the LAL Reagent and Chromogenic Substrate?

    Each vial of LAL Reagent contains 1.1 ml, and each vial of Chromogenic Substrate contains 2.2 ml. There are 2 vials of each provided in the kit.