End-point Chromogenic Method for Bacterial Endotoxin Testing: China Suppliers and Factory Solutions

End-point Chromogenic Method for Bacterial Endotoxin Testing (BET)
The End-point Chromogenic Method is a reliable and quantitative assay that utilizes Limulus Amebocyte Lysate (LAL). This advanced method enables the detection of endotoxins through the activation of Factor C, which initiates an enzymatic cascade that cleaves a chromogenic substrate, resulting in the release of a measurable chromophore. It is essential to note that this product is not registered with the U.S. FDA and is intended for research use only in the U.S.
Procedure:
To perform the test, simply mix the test sample with LAL reagent and chromogenic substrate. Incubate the mixture at 37 ± 1 °C for a specified duration (e.g., 60 minutes). After incubation, stop the reaction using acid and measure the absorbance at 405 nm. The endotoxin levels in your sample are determined by comparing the absorbance against a standard curve created from known endotoxin concentrations. This method offers exceptional specificity and quantitative accuracy, making it a preferred choice in pharmaceutical and medical device testing. As a leading manufacturer and supplier of this testing method, we are proud to serve clients across China and beyond, ensuring high-quality standards in every analysis