End-point Chromogenic Method for Bacterial Endotoxin Testing - China Suppliers and Factory Solutions

End-point Chromogenic Method for Bacterial Endotoxin Testing (BET)
The End-point Chromogenic Method is a highly effective quantitative assay based on Limulus Amebocyte Lysate (LAL). This method utilizes the activation of Factor C by endotoxin, which initiates an enzymatic cascade that cleaves a chromogenic substrate, thereby releasing a measurable chromophore for accurate detection. This product is not registered with the U.S. FDA. For use in the U.S., it is intended for research purposes only.
Procedure:
Mix the test sample with LAL reagent and chromogenic substrate. Incubate at 37 ± 1 °C for a specified duration (e.g., 60 minutes). Stop the reaction with acid and measure the absorbance at 405 nm. Endotoxin levels are quantified by comparing the sample’s absorbance to a standard curve created from known endotoxin concentrations. This method offers high specificity and quantitative accuracy, making it a preferred choice among suppliers and manufacturers in the pharmaceutical and medical device sectors, especially in China. As a reliable factory for endotoxin testing solutions, we ensure the utmost quality and efficiency for your testing needs